If you were wondering how proteins run in a polyacrylamide gel, here’s a video! SDS-PAGE is the first step of westernblot, the proteins are reduced and negatively charged, so they run in a measure proportional to their molecular weight. The molecular weight marker is a mix of known proteins used to determine the weight of protein of interest. Once the run finish, the proteins are transferred into a nitrocellulose membrane. Follow @biotechnologyguy For science blogs and Daily Science Updates✉️. #
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